TruArray®
Sequence Template Guidelines
Template File Versions
File Format
The file is an Excel file with two sheets. You can rename the file, but please do not modify the sheet names.
Oligo_ID_and_Seq Sheet
The sheet records the names and sequences of oligonucleotides, containing three columns of information
The index for each oligonucleotide
The name of each oligonucleotide
The sequence of each oligonucleotide
Array_Coord Sheet
The sheet sets the coordinates of the oligonucleotides on the chip, containing three columns of information
The row coordinates on the chip
The column coordinates on the chip
The index number of each oligonucleotide
Filling and Validation Rules
Oligo_ID_and_Seq Sheet
- Must be continuous, non-repeating positive integers.
- Should not be modified.
- If you do not wish to set names, you may leave the entire column blank (IDs will be assigned automatically); however, you can not fill some rows and leave others blank.
- Duplicate Oligo_IDs are not allowed. If a sequence needs to be synthesized multiple times, please assign multiple coordinates in the Array_Coord sheet instead of duplicating the row.
- Allowed characters: Uppercase and lowercase letters (A-Z, a-z), Arabic numerals (0-9), and certain symbols (+-=_.:). Spaces, full-width characters (including Chinese), line breaks, tabs, and other special characters are prohibited.
- Due to limitations in most bioinformatics software, the first character of the Oligo_ID must be a letter (A-Z, a-z) and cannot be a number or special character.
- Allowed characters: Letters (A-Z, a-z) and Arabic numerals (0-9).
- Unless there are special requirements, use uppercase A, C, G, T for natural bases. By default, lowercase letters will be converted to uppercase.
- Uppercase R, Y, S, W, K, M, B, D, H, V, N represent condensed bases. By default, lowercase letters will be converted to uppercase.
- You may use N for random condensed bases. However, if you need to synthesize non-N condensed bases (e.g., R, Y, S, W, K, M, B, D, H, V), please contact our customer manager for clarification and confirmation.。
- Characters other than A, C, G, T, R, Y, S, W, K, M, B, D, H, V, N (including numbers) may represent special monomers, specific synthesis recipe, or modification groups. If required, please contact our customer manager.
- If your lower- and uppercase letters represent different monomers or recipes, please contact our customer manager.
Array_Coord Sheet
- Must be continuous, non-repeating positive integers.
- Should not be modified.
- Must be continuous, non-repeating positive integers.
- Should not be modified.
- Must be a positive integer, left blank, or kept as “Blocked for QC Loci”.
- Leaving it blank indicates that no oligonucleotide synthesis will be performed at that coordinate.
- Keeping “Blocked for QC Loci” indicates that the coordinate is reserved for synthesizing quality control sequences.
- A positive integer represents the synthesis of a custom user sequence from the Oligo_ID_and_Seq sheet. This value must exist in the Oligo_No column of the Oligo_ID_and_Seq sheet, and the corresponding Oligo_Seq value for that row must not be empty.
Additional Suggestions and Rules
In the Oligo_ID_and_Seq sheet, each row represents one unique oligonucleotide sequence. For example, if a user needs to synthesize 1,800 different sequences using a 4K chip with 2 copies of each synthesized at different coordinates on the chip (occupying a total of 3,600 pixels), they should fill rows with Oligo_No 1 to 1,800 in the Oligo_ID_and_Seq sheet, and then assign two coordinates (two rows) for each sequence in the Array_Coord sheet, rather than filling rows with Oligo_No 1 to 3,600 in the Oligo_ID_and_Seq sheet.
The row where Oligo_No is “0” and Oligo_ID is “Demo_Oligo” is an example row. Please do not modify it. This row is not synthesized and will not appear in the final product.
Sequences that are defined in the Oligo_ID_and_Seq sheet but are not referenced in the Oligo_No column of the Array_Coord sheet will not be synthesized. We recommend that you verify these entries.
In principle, Oligo_Seq should correspond one-to-one with Oligo_ID. A single Oligo_ID can only correspond to one Oligo_Seq. While different Oligo_IDs corresponding to the same Oligo_Seq are permitted, we recommend verifying your design.
Avoid cases where Oligo_ID is empty while Oligo_Seq contains data.
