An oligonucleotide pool, or oligo pool, is a mixed library of hundreds to millions of customized oligonucleotides.
Oligo pools are manufactured and normally delivered as single-stranded oligonucleotides.
Oligo pools can also be amplified, error-corrected, and delivered as double-stranded DNA products.
Or cloned into universal or customized vectors and delivered as pooled plasmid libraries.
An oligonucleotide pool, or oligo pool, is a mixed library of hundreds to millions of customized oligonucleotides. Oligo pools are manufactured and normally delivered as single-stranded oligonucleotides. Oligo pools can also be amplified, error-corrected, and delivered as double-stranded DNA products. Or cloned into universal or customized vectors and delivered as pooled plasmid libraries.
Highlights
Throughput flexibility
- Multiple throughput specifications of TFT-DNA chips to support customization of different pool sizes
Throughput-yield balance
- Higher per-oligo yield than commercially available CMOS electrochemical and inkjet-printed DNA synthesis routes
High precision [1] [2]
- Coverage up to >99.9%
- Error rate down to <0.4%/nt
- World-leading electrochemical DNA synthesis quality
High uniformity [1] [2] [3]
- Pre-amplificationQ95:Q5 < 2.0X
- Post-amplificationQ95:Q5 down to < 3.0X
Support for sequence customization [4]
- Support for degenerate bases
- Support for special monomers
- Support for 5′ modifications
Support for subpool segmentation [4]
- 4K oligo pool can be divided into up to 16 subpools, with 256 oligos in each subpool
- 59K oligo pool can be divided into up to 16 subpools, with 3,712 oligos in each subpool
Application Scenarios
- Targeted NGS capture probes
- Gene variant libraries
- High-throughput antibody discovery
- High-throughput aptamer discovery
- AI-assisted protein design
- CRISPR gRNA screening libraries
- Lentiviral shRNA screening libraries
- Factory-scale gene synthesis
- Germplasm development and identification
- DNA data storage
Product Category
|
Product Category |
Delivery Form |
Available Customization |
Yield per Oligo |
Oligo Length |
Pool Size |
Turnaround Time |
|
TruPool® Std |
• ssDNA, unamplified • ssXNA, unamplified [4] |
• 3'-OH • 5'-modification [4] • Condensed base [4] • Special monomer [4] |
Femtomole scale [5] |
30~ 150nt [6] |
• 4K Chip: Up to 4,096 oligos per chip • 4K Partitioned Chip: Up to 4,096 oligos and 16 subpools per chip • 65K Chip: Up to 65,536 oligos per chip • 59K Partitioned Chip: Up to 59,392 oligos and 16 subpools per chip • 16K/32K/120K/0.5M Chip: Coming soon [4] |
5~15 working days |
|
TruPool® dsMax |
dsDNA, amplified |
• Amplification by subpools[4] • Amplification according to chip partitions [4] |
Picomole to nanomole scale |
8~20 working days |
||
|
TruPool® Clonal [4] |
Pooled vectors |
• Common vector [4] • Customized vector [4] |
| Product Category | TruPool® Std | TruPool® dsMax | TruPool® Clonal [4] |
| Delivery Form |
• ssDNA, unamplified • ssXNA, unamplified [4] |
dsDNA, amplified | Pooled vectors |
| Available Customization |
• 3'-OH •5'-modification [4] • Condensed base [4] • Special monomer [4] |
• Amplification by subpools[4] • Amplification according to chip partitions [4] |
• Common vector [4] • Customized vector [4] |
| Yield per Oligo | Femtomole scale [5] | Picomole to nanomole scale | |
| Oligo Length | 30~150nt [6] | ||
| Pool Size |
• 4K Chip: Up to 4,096 oligos per chip • 4K Partitioned Chip: Up to 4,096 oligos and 16 subpools per chip • 65K Chip: Up to 65,536 oligos per chip • 59K Partitioned Chip: Up to 59,392 oligos and 16 subpools per chip • 16K/32K/120K/0.5M Chip: Coming soon [4] |
||
| Turnaround Time | 5~15 working days | 8~20 working days | |
Specifications
| Oligo Pool Size | Up to 4,096 or 59,392/65,536 per chip |
 |
| Subpool Number and Size |
• 4K chip supports 16 subpools, each containing up to 256 oligos • 59K chip supports 16 subpools, each containing up to 3,712 oligos |
|
| Oligo Length | Up to 150 nt | |
| Coverage | >99.5% | |
| Error Rate | < 0.8% / nt | |
| Pre-amplification Uniformity |
• > 90% of oligos represented within < 3.0X of the median • > 99% of oligos represented within < 5.0X of the median |
|
| Available Modification |
• 3'-OH • 5'-phosphorylation |
|
| Available Special Monomers |
• Condensed base • Special monomer |
|
| Turnaround Time | 5~15 working days |
| Oligo Pool Size | Up to 4,096 or 59,392/65,536 per chip |
| Subpool Number and Size |
• 4K chip supports 16 subpools, each containing up to 256 oligos • 59K chip supports 16 subpools, each containing up to 3,712 oligos |
| Oligo Length | Up to 150 nt |
| Coverage | >99.5% |
| Error Rate | < 0.8% / nt |
| Pre-amplification Uniformity |
• > 90% of oligos represented within < 3.0X of the median • > 99% of oligos represented within < 5.0X of the median |
| Available Modification |
• 3'-OH • 5'-phosphorylation |
| Available Special Monomers |
• Condensed base • Special monomer |
| Turnaround Time | 5~15 working days |
TruPool® Ordering Process
Place Order
Confirmation
Production
Quality Control
Delivery
Place
Order
Confir-
mation
Produc-
tion
Quality
Control
