TruSynth®
Oligonucleotide Microarray
Synthesizer
World's first oligonucleotide microarray synthesizer
based on TFT semiconductor technology
TruSynth®
Oligonucleotide Microarray Synthesizer
World's first oligonucleotide microarray synthesizer based on TFT semiconductor technology
LinkZill's TruSynth® oligonucleotide microarray synthesizer achieves high-throughput, high-precision synthesis of oligonucleotide microarrays and oligo pools by precisely controlling the electrical signals of arrayed pixels on a proprietary TFT chip to drive thousands to millions of individual electrochemical reactions.
The TruSynth® synthesizer has achieved world-leading electrochemical high-throughput DNA synthesis specifications.
The TruSynth® Moore series supports additional RNA monomers, non-natural nucleotide monomers, modifications, and premixed condensed base monomers, enabling the decentralized synthesis of XNA oligonucleotide microarrays and pools.
LinkZill's TruSynth® oligonucleotide microarray synthesizer achieves high-throughput, high-precision synthesis of oligonucleotide microarrays and oligo pools by precisely controlling the electrical signals of arrayed pixels on a proprietary TFT chip to drive thousands to millions of individual electrochemical reactions.
The TruSynth® synthesizer has achieved world-leading electrochemical high-throughput DNA synthesis specifications.
The TruSynth® Moore series supports additional RNA monomers, non-natural nucleotide monomers, modifications, and premixed condensed base monomers, enabling the decentralized synthesis of XNA oligonucleotide microarrays and pools.
Highlights
End-user accessibility
- A high-throughput oligonucleotide microarray synthesizer that sells to end users
Throughput flexibility
- Adaptable to 4K- or 59K/65K-throughput TFT-DNA synthesis chip
- Upgradeable to mega-scale chips in the future
Flexible partitioning
- 4K and 59K chips with 16 partitions are available
- Partitioning schemes are customizable
Throughput-yield balance
- Higher per-oligo yield than commercially available CMOS electrochemical and inkjet-printed DNA synthesis routes
World-leading product quality [1] [2]
- Coverage up to > 99.9%
- Error rate down to < 0.4% / nt
- World-leading electrochemical DNA synthesis quality
Support for customization [3]
- Support for condensed bases, special monomers, and 5′ modifications
- Support for chip design and pattern customization
Recipe flexibility
- Built-in high-efficiency, high-precision DNA synthesis recipe
- Support for customized recipe
Extremely rapid production
- Single synthesis cycle duration of only 5 minutes
- Built-in algorithms to minimize synthesis time for long oligonucleotides
- 150 nt oligonucleotide microarray synthesis in 34 hours
Compact, easy-to-deploy, and easy-to-use device
- Bench-top device with a footprint of only 0.3 ㎡
- Fully enclosed synthesis system with low environmental requirements such as humidity, air pressure, vibration, etc.
- Relatively simple mechanical structure, easy to maintain
- User-friendly interface for non-professionals, easy to operate
Low consumable and reagent cost
- Lower chip cost than CMOS-based technologies
- Micro-scaled reaction system
- Compatible with standard chemical synthesis reagents, reagent cost as low as ¥1.0 per cycle
Application Scenarios
High-throughput omics technology
- Targeted NGS capture probes
- Genomic variant genotyping
- Gene expression profiling
- In situ spatial OMICs
- Solid-phase capture
- Non-mass-spectrometric proteomics
- Sequencing technology
Synthetic biology
- Gene mutagenesis libraries
- Factory-scale gene synthesis
- AI-assisted protein design
- In situ gene synthesis
- DNA data storage
Innovative drug discovery
- CRISPR gRNA screening libraries
- Lentiviral shRNA screening libraries
- High-throughput antibody discovery
- High-throughput aptamer discovery
Medical diagnostics
- Pathogen and resistance screening
- Pharmacogenetics
- Tumer genotyping
- MRD monitoring
Molecular breeding and agriculture
- Animal germplasm identification
- Plant germplasm identification
- Genome-editing-based germplasm development
Technical Specifications [1] [2] [4]
Coupling Efficiency: > 98.5%
Array Size: Up to 4,096 or 59,392/65,536 per chip
Partition Number and Size:
4K chip supports 16 partitions, each containing up to 256 oligos
59K chip supports 16 partitions, each containing up to 3,712 oligos
Oligo Length: Up to 150 nt, unlockable up to 200 nt [5]
Coverage: > 99.5%
Synthetic Uniformity [4] :
Pre-amplification: Q95:Q5 < 2.0X
Post-amplification: Q95:Q5 < 4.0X
Error Rate: < 0.8% / nt
Synthesis Yield: > 0.5 fmol per oligo [6]
Single Synthesis Cycle Duration:
Default recipe: 5.5 min
Custom recipe can be adjusted to: < 3.5 min
Production Time [7] :
60 nt: approx. 9 hr
100 nt: approx. 20 hr
150 nt: approx. 34 hr
Consumables and Synthesis Products
Device Specifications
| Device Model |
 |
 |
|||
| TruSynth® Base | TruSynth® Moore | ||||
| # of Chips per Synthesis Task | 1 | ||||
| Chip Compatibility |
 |
 |
 |
 |
|
| 4K Partitioned | 4K Unpartitioned | 59K Partitioned | 65K Unpartitioned | ||
| Appearance | W × L × H | 518mm × 511mm × 380mm | 670mm × 620mm × 560mm | ||
|
Net Weight（Excluding Reagent Bottles and Accessories） |
40.0kg | 60.0kg | |||
| PC | Computer + Monitor | External | External | ||
|
Working Environment |
Temperature | 20℃～30℃ | |||
| Relative Humidity | 10%～45% | ||||
|
Supported Monomer Species |
Conventional Base Monomer | 500 mL × 4 | 500 mL × 4 | ||
|
Special Base Monomer or Modification Reagent |
0 | 500 mL × 1 + 25–50 mL × 5 | |||
| Power Supply | Voltage | 100~240V AC | |||
| Frequency | 50/60Hz | ||||
| Rated Power | 48W | 68W | |||
| Device Model | TruSynth® Base | TruSynth® Moore | |
| # of Chips per Synthesis Task | 1 | ||
| Chip Compatibility | 4K Partitioned / 4K Unpartitioned / 59K Partitioned / 65K Unpartitioned | ||
| Appearance | W × L × H | 518 mm × 511 mm × 380 mm | 670 mm × 620 mm × 560 mm |
| Net Weight（Excluding Reagent Bottles and Accessories） | 40.0 kg | 60.0 kg | |
| PC | Computer + Monitor | External | External |
| Working Environment | Temperature | 20℃～30℃ | |
| Relative Humidity | 10%～45% | ||
| Supported Monomer Species | Conventional Base Monomer | 500 mL × 4 | 500 mL × 4 |
| Special Base Monomer or Modification Reagent | 0 | 500 mL × 1 + 25–50 mL × 5 | |
| Power Supply | Voltage | 100~240V AC | |
| Frequency | 50/60Hz | ||
| Rated Power | 48W | 88W | |
Ordering Information
| Product Category | Product Name | Model | Specification |
| Microarray Synthesizer | TruSynth® Oligonucleotide Microarray Synthesizer Base-4K | Base-4K | 1 set with external PC and monitor |
| TruSynth® Oligonucleotide Microarray Synthesizer Base-65K | Base-65K | ||
| TruSynth® Oligonucleotide Microarray Synthesizer Moore-4K | Moore-4K | ||
| TruSynth® Oligonucleotide Microarray Synthesizer Moore-65K | Moore-65K | ||
| Interchangeable Drive System [8] | TruSynth® 4K TFT-DNA chip drive system | Driver-4K | 1 set |
| TruSynth® 59K/65K TFT-DNA chip drive system | Driver-59K/65K | ||
| TFT-DNA Synthesis Chip | TFT-DNA Chip 4K Type-A Unpartitioned | Std-4K-A |
1 piece 75.0mm × 25.4mm × 0.5mm |
| TFT-DNA Chip 4K Type-B w/ 16 Partitions [9] | Std-4K-B | ||
| TFT-DNA Chip 65K Type-A Unpartitioned | Std-65K-A | ||
| TFT-DNA Chip 59K Type-B w/ 16 Partitions [9] | Std-59K-B | ||
| Proprietary Reagent | Electrochemical dimethoxyphenyl trimethyl group (DMT) remover | EGA-AC02-RDE | 60.5g [6] |
| Other exclusively improved synthetic reagents [5] | |||
| Replaceable Accessories | Chip flowcell [5] | ||
| Solenoid / Valve group / Pipeline [5] | |||
| Optional Supporting Equipment | Six-channel reagent dissolver | ||
| Gas-phase ammonolysis system compatible with TFT chips | |||
| Elution module for 59K partitioned chip |
| Product Category | Product Name | Model | Specification |
| Microarray Synthesizer | TruSynth® Oligonucleotide Microarray Synthesizer Base-4K | Base-4K | 1 set with external PC and monitor |
| TruSynth® Oligonucleotide Microarray Synthesizer Base-59K/65K | Base-59K/65K | ||
| TruSynth® Oligonucleotide Microarray Synthesizer Moore-4K | Moore-4K | ||
| TruSynth® Oligonucleotide Microarray Synthesizer Moore-59K/65K | Moore-59K/65K | ||
| Interchangeable Drive System [8] | TruSynth® 4K TFT-DNA chip drive system | Driver-4K | 1 set |
| TruSynth® 59K/65K TFT-DNA chip drive system | Driver-59K/65K | ||
| TFT-DNA Synthesis Chip | TFT-DNA Chip 4K Type-A Unpartitioned | Std-4K-A | 1 piece （75.0 mm × 25.4 mm × 0.5 mm） |
| TFT-DNA Chip 4K Type-B w/ 16 Partitions [9] | Std-4K-B | ||
| TFT-DNA Chip 65K Type-A Unpartitioned | Std-65K-A | ||
| TFT-DNA Chip 59K Type-B w/ 16 Partitions [9] | Std-59K-B | ||
| Proprietary Reagent | Electrochemical dimethoxyphenyl trimethyl group (DMT) remover | EGA-AC02-RDE | 60.5g [6] |
| Other exclusively improved synthetic reagents [5] | |||
| Replaceable Accessories | Chip flowcell [5] | ||
| Solenoid / Valve group / Pipeline [5] | |||
| Optional Supporting Equipment | Six-channel reagent dissolver | ||
| Gas-phase ammonolysis system compatible with TFT chips | |||
| Elution module for 59K partitioned chip |
