An oligo pool is a mixed library of hundreds to millions of customized oligonucleotides.
Oligo pools are manufactured and normally delivered in the form of single-strand oligonucleotides.
Also, oligo pools can be amplified, error-corrected, and delivered as double-stranded DNA products.
Or cloned into universal or customized vectors, and delivered as pooled plasmid libraries.
An oligo pool is a mixed library of hundreds to millions of customized oligonucleotides. Oligo pools are manufactured and normally delivered in the form of single-strand oligonucleotides. Also, oligo pools can be amplified, error-corrected, and delivered as double-stranded DNA products. Or cloned into universal or customized vectors, and delivered as pooled plasmid libraries.
Highlights
Throughput flexibility
- Multiple throughput specifications of TFT-DNA chips to support customization of different pool sizes
Throughput-yield balance
- Higher per oligo yield than commercially available CMOS electrochemical and inkjet printed DNA synthesis routes
High precision [1] [2]
- Coverage up to >99.9%
- Error rate down to <0.6%/nt
- World-leading electrochemical DNA synthesis quality
High uniformity [1] [2] [3]
- Pre-amplification Q95:Q5<2.0X
- Pre-amplification Q99.5:Q0.5<4.0X
- Q95:Q5 down to <4.0XPost-amplification
Support for sequence customization [4]
- Support for degenerate bases
- Support for special monomers
- Support for 5′ modifications
Application Scenarios
- Targeted NGS capture probes
- Gene variant libraries
- High-throughput antibody discovery
- High-throughput aptamer discovery
- AI-assisted protein design
- CRISPR gRNAs screening libraries
- Lentiviral shRNA screening libraries
- Factory-scale gene synthesis
- Germplasm development and identification
- DNA data storage
Product Category
|
Product Category |
Delivery Form |
Available Customization |
Yield per Oligo |
Oligo Length |
Pool Size |
Turn-around Time |
|
TruPool® Std |
ssDNA, unamplified [5] |
• 3'-OH •5'-phosphorylation [4] • Degenerate base [4] • Special monomer [4] |
Femtomole scale [6] |
30~ 150nt [7] |
• 4K Chip: Up to 4,096 per chip • 65K Chip: Up to 65,536 per chip • 0.5M Chip: Coming soon [8] |
5~15 working days |
|
TruPool® dsMax |
dsDNA, amplified |
- | Picomole to nanomole scale |
8~20 working days |
||
|
TruPool® Clonal [9] |
Pooled vectors |
| Product Category | TruPool® Std | TruPool® dsMax | TruPool® Clonal [9] |
| Delivery Form | ssDNA, unamplified [5] | dsDNA, amplified | Pooled vectors |
| Available Customization |
• 3'-OH •5'-phosphorylation [4] • Degenerate base [4] • Special monomer [4] |
- | |
| Yield per Oligo | Femtomole scale [6] | Picomole to nanomole scale | |
| Oligo Length | 30~150nt [7] | ||
| Pool Size |
• 4K Chip: Up to 4,096 per chip • 65K Chip: Up to 65,536 per chip • 0.5M Chip: Coming soon [8] |
||
| Turn-around Time | 5~15 working days | 8~20 working days | |
Specifications
| Oligo Pool Size | Up to 4,096 or 65,536 per chip |
 |
| Oligo Length | Up to 150 nt | |
| Coverage | >99.0% | |
| Error Rate | < 1.0%/nt | |
| Pre-amplification Uniformity |
• > 90% of oligos represented within < 3.0X of the median • > 99% of oligos represented within < 5.0X of the median |
|
| Available Modification |
• 3'-OH • 5'-phosphorylation |
|
| Available Special Monomers |
• Degenerate base • Special monomer |
|
| Turn-around Time | 5~15 working days |
| Oligo Pool Size | Up to 4,096 or 65,536 per chip |
| Oligo Length | Up to 150 nt |
| Coverage | >99.0% |
| Error Rate | < 1.0% / nt |
| Pre-amplification Uniformity |
• > 90% of oligos represented within < 3.0X of the median • > 99% of oligos represented within < 5.0X of the median |
| Available Modification |
• 3'-OH • 5'-phosphorylation |
| Available Special Monomers |
•Degenerate base • Special monomer |
| Turn-around Time | 5~15 working days |
TruPool® Ordering Process
Place Order
Confirmation
Production
Quality Control
Delivery
Place
Order
Confir-
mation
Produc-
tion
Quality
Control
